肿瘤细胞治疗研究
英文原题：Variability of the Immunosuppressive Properties of Glioma Cell Lines.
Variability of the Immunosuppressive Properties of Glioma Cell Lines.
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免疫治疗方法，如使用检查点抑制剂、CAR-T 细胞、树突状细胞疫苗等，为胶质母细胞瘤的治疗提供了有前景的途径。用于胶质瘤治疗的树突状细胞（DC）疫苗已被广泛研究，并在某些情况下有效。然而，尽管DC疫苗用于胶质瘤患者的治疗显示出有前景的结果，但仍受限于肿瘤抗原及其负载方法的选择欠佳，以及肿瘤细胞微环境的免疫抑制性质。在这项工作中，我们表明胶质瘤细胞系表达广谱免疫检查点，在直接共培养过程中抑制同种异体DC的成熟，并且不刺激同种异体淋巴细胞的增殖。用负载相应肿瘤裂解物的树突状细胞致敏的淋巴细胞仅有效裂解了三种胶质瘤细胞系中的两种，T98G和U373 MG，而1321N1细胞完全抑制了通过与负载这些细胞裂解物的DC共培养致敏的淋巴细胞的细胞毒活性。因此，DC疫苗的疗效可能与胶质瘤细胞的个体特征有关。
Immunotherapeutic methods, such as the use of checkpoint inhibitors, CAR T cells, dendritic cell vaccines, etc. , offer promising approach to the treatment of glioblastomas. Dendritic cell (DC)-based vaccines used in glioma therapy have been well studied and are effective in some cases.
However, the use of DC vaccines for the treatment of patients with gliomas, though shows promising results, is still limited by the suboptimal choice of tumor antigen and the method of its loading, as well as the immunosuppressive nature of the tumor cell microenvironment. In this work, we showed that glioma cell lines express a broad spectrum of immune checkpoints, suppress the maturation of allogeneic DCs during direct co-culturing, and do not stimulate the proliferation of allogeneic lymphocytes.
Lymphocytes primed with dendritic cells loaded with the corresponding tumor lysates effectively lysed only two of three glioma cell lines, T98G and U373 MG, whereas 1321N1 cells completely suppressed the cytotoxic activity of lymphocytes primed by co-culturing with DCs loaded with the lysate of these cells.
Thus, the efficacy of DC vaccines may be related to the individual characteristics of glioma cells.
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