不适合移植的大 B 细胞淋巴瘤二线使用 axicabtagene ciloleucel：ALYCANTE 最终分析
Second-line axicabtagene ciloleucel in large B-cell lymphoma ineligible for transplantation: ALYCANTE final analysis.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
Smo 基因沉默：通过调控增殖和凋亡治疗自然杀伤/T 细胞淋巴瘤的一种有前景的策略
英文原题：Smo gene silencing: a promising strategy for natural killer/t-cell lymphoma treatment via modulating proliferation and apoptosis.
查看英文原题
Smo gene silencing: a promising strategy for natural killer/t-cell lymphoma treatment via modulating proliferation and apoptosis.
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中文摘要
自然杀伤/T细胞淋巴瘤（NKTCL）是一种预后不良的恶性肿瘤。Smoothened（Smo）蛋白与NKTCL的生长有关。本研究采用慢病毒载体介导的Smo RNA干扰（LV-Smo-RNAi）沉默人NKTCL细胞系SNT8中的Smo基因。荧光显微镜、qRT-PCR和Western blot验证了Smo mRNA和蛋白水平的降低。CCK-8实验显示，Smo沉默抑制了细胞增殖。annexin V-PE/7-AAD双染流式细胞术表明凋亡率增加。此外，GLI家族锌指1（Gli1）和程序性死亡配体1（PD-L1）的表达下调。在体内，异种移植实验表明，Smo沉默导致肿瘤生长减慢，肿瘤体积和重量减少。总体而言，Smo基因沉默通过有效抑制细胞增殖和促进凋亡，有望成为NKTCL的一种新型分子靶向治疗方法。
展开英文摘要原文
Natural killer/T-cell lymphoma (NKTCL) is a malignancy with a poor prognosis. The Smoothened (Smo) protein is implicated in NKTCL growth.
This study employed lentiviral vector-mediated Smo RNA interference (LV-Smo-RNAi) to silence the Smo gene in the human NKTCL cell line SNT8. Fluorescence microscopy, qRT-PCR, and Western blot verified the reduction of Smo mRNA and protein levels. The CCK-8 assay showed that Smo silencing inhibited cell proliferation. Flow cytometry with annexin V-PE/7-AAD double staining indicated an increased apoptosis rate.
Moreover, the expression of GLI family zinc finger 1 (Gli1) and programmed death-ligand 1 (PD-L1) was downregulated. In vivo, xenotransplantation experiments demonstrated that Smo silencing led to slower tumor growth with reduced tumor volume and weight.
Overall, Smo gene silencing holds great potential as a novel molecular-targeted therapy approach for NKTCL by effectively suppressing cell proliferation and promoting apoptosis.
论文信息
- 作者
- Liu S、Wang S、Xu Y、Huang Y、Xin P、Zheng Y、Wu Y、Yang Y
- 第一作者单位
- Department of Hematology, First Hospital of Quanzhou Affiliated to Fujian Medical University, 248 East Street, Licheng District, Quanzhou, 362000, Fujian Province, China.China
- 通讯作者单位
- Department of Hematology, First Hospital of Quanzhou Affiliated to Fujian Medical University, 248 East Street, Licheng District, Quanzhou, 362000, Fujian Province, China. lct090909@163.com.China
- 文献类型
- 非美国政府资助研究
- 期刊
- Molecular medicine (Cambridge, Mass.)2025 Oct 29
- 原文标识
- PubMed 41162883 · DOI 10.1186/s10020-025-01341-z
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