CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
采用流式细胞术评估自体 2D 共培养中的功能性抗肿瘤 T 细胞应答
英文原题：Evaluating functional anti-tumor T cell responses in autologous 2D co-cultures using flow cytometry.
查看英文原题
Evaluating functional anti-tumor T cell responses in autologous 2D co-cultures using flow cytometry.
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中文摘要
T细胞介导的免疫监视是癌症进展的关键决定因素，基于T细胞的免疫疗法作为癌症患者的治疗方式已展现出巨大前景。因此，评估T细胞与肿瘤细胞之间相互作用的生理相关方法尤为受到关注。能够在自体TIL（肿瘤浸润淋巴细胞）存在下培养患者来源肿瘤细胞的体外系统，是理解T细胞在癌症中的基本生物学作用以及如何调控其功能以获得治疗获益的宝贵工具。因此，本章描述了一种基于流式细胞术的方法，用于评估培养中暴露于自体肿瘤细胞后TIL的活化。具体而言，本章将重点介绍在此类培养系统中评估细胞毒性淋巴细胞（CTL）脱颗粒和分泌促炎细胞因子能力的方法。
展开英文摘要原文
Immune surveillance by T cells is a key determinant of cancer progression, and T cell-based immunotherapies have shown great promise as a treatment modality for cancer patients. As such, physiologically relevant methods to evaluate interactions between T cells and tumor cells are of particular interest. In vitro systems that enable the culture patient-derived tumor cells in the presence of autologous tumor-infiltrating lymphocytes (TIL) serve as an invaluable tool to understand the basic biological role of T cells in cancer and how their functioning might be modulated to gain therapeutic benefit.
Hence, this chapter describes a flow cytometry-based approach to assess TIL activation by exposure to autologous tumor cells in culture. In particular, the chapter will focus on ways to assess the capacity of cytotoxic lymphocytes (CTL) to degranulate and secrete pro-inflammatory cytokines in such culture systems.
论文信息
- 作者
- Baldran-Groves L、Melief J、Lundqvist A
- 单位
- Department of Oncology-Pathology, Karolinska Institutet, Solna, Sweden.Sweden
- 期刊
- Methods in cell biology2025
- 原文标识
- PubMed 40683674 · DOI 10.1016/bs.mcb.2025.01.002