乳酸脱氢酶 B 促进肿瘤浸润 CD8(+) T 细胞的二硫死亡和耗竭

The aberrant accumulation of intracellular disulfides promotes cancer cell disulfidptosis; however, how disulfide stress influences tumour-infiltrating CD8 + T cell function remains unknown.

Here we demonstrate that lactate dehydrogenase B (LDHB) facilitates intratumoural CD8 + T cell disulfidptosis and exhaustion, leading to impaired antitumour immunity. SLC7A11-mediated cystine uptake by CD8 + T cells induces disulfidptosis, which plays critical roles in the development of exhausted CD8 + T cells.

LDHB restricts glucose-6-phosphate dehydrogenase (G6PD) activity in exhausted CD8 + T cells by interacting with G6PD, causing NADPH depletion and consequently triggering disulfidptosis. Accordingly, the loss of LDHB in T cells prevents disulfidptosis-dependent CD8 + T cell exhaustion and improves antitumour immunity.

Mechanistically, STAT3 directs LDHB expression to limit G6PD activity and mediate disulfidptosis in exhausted CD8 + T cells.

Our results highlight the distinct roles of disulfidptosis and ferroptosis in driving CD8 + T cell exhaustion and suggest a potential therapeutic strategy to target LDHB in cancer immunotherapy.