CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
英文原题：Protocol for assessing mobilization of peritoneal B cells to the pre-metastatic omentum in an orthotopic mouse model of ovarian cancer.
Protocol for assessing mobilization of peritoneal B cells to the pre-metastatic omentum in an orthotopic mouse model of ovarian cancer.
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大网膜是一种内脏脂肪组织，在转移发生前后会经历动态的免疫学变化。在此，我们提出了一种在小鼠卵巢癌模型中评估腹膜B细胞向转移前大网膜动员的方案。我们描述了腹膜供体B细胞的分离和过继转移及其在受体小鼠大网膜中检测的步骤。该方案可用于研究其他病理背景下腹膜B细胞向大网膜的动员。关于本方案使用和执行的完整细节，请参阅Lee et al. 1。
The omentum is a visceral adipose tissue that undergoes dynamic immunological changes prior to and following metastasis.
Here, we present a protocol for assessing the mobilization of peritoneal B cells to the pre-metastatic omentum in a mouse ovarian cancer model.
We describe steps for isolation and adoptive transfer of peritoneal donor B cells and their detection in the omentum of recipient mice. This protocol could be utilized to study the mobilization of peritoneal B cells to the omentum in other pathological contexts. For complete details on the use and execution of this protocol, please refer to Lee et al. 1 .
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