两种化疗药物在抗肿瘤免疫反应中扩增干细胞样 CD62L(+)CD8(+) T 细胞

Tpex cell-associated subpopulations in human colorectal tumors were analyzed by using single-cell sequencing data. CT26 and B16 tumor models of wild type and Eomes conditional knockout mice were constructed, and the changes of T TSM , Tpex and Tex subsets in mice were dissected by flow cytometry after treatment with decitabine (DAC), doxorubicin (DOX) and 5-Fluorouracil (5-FU).

In this study, we demonstrated that DAC and 5-FU expanded CD8 + T TSM cells in TdLNs. At the same time, we validated that DAC and 5-FU substantially promoted the expansion of CD62L + CD8 + Tpex cells and subsequently increased effector function of CX3CR1 + CD8 + Tex int cells. In addition, the conditional knockout of transcription factor Eomes in CD8 + T cells partially eliminated DAC-amplified CD62L + CD8 + Tpex cells, but had no effect on such CD8 + T subset expanded by 5-FU.

The present study demonstrated that both DAC and 5-FU promoted the differentiation of stem-like CD8 + T TSM cells in TdLNs and significantly enhanced the differentiation and expansion of stem-like CD62L + CD8 + Tpex and CX3CR1 + Tex int cells in tumor microenvironment. The knockout of Eomes partially influenced the role of DAC in promoting the differentiation and expansion of stem-like CD8 + T cells.