CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
研究转录调节因子缺陷通过过继性细胞转移对肿瘤特异性 CD8(+) T 细胞应答影响的方案
英文原题：Protocol for investigating the impact of transcription regulator deficiency on tumor-specific CD8(+) T cell responses via adoptive cell transfer.
查看英文原题
Protocol for investigating the impact of transcription regulator deficiency on tumor-specific CD8(+) T cell responses via adoptive cell transfer.
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中文摘要
转录因子在肿瘤特异性CD8+ T细胞的分化中起关键作用，并显著影响其抑制肿瘤的能力。在此，我们提出了一种方案，用于在肿瘤特异性CD8+ T细胞中通过他莫昔芬诱导创建DNA 3（ID3）转录调节因子抑制剂的条件性敲除小鼠。我们描述了通过共过继转移实验检测ID3缺失对肿瘤引流淋巴结中肿瘤特异性记忆CD8+ T细胞（Ttsm）和耗竭CD8+ T细胞祖细胞（Tpex）分化影响的步骤。有关本方案使用和执行的完整详细信息，请参阅Ran等1。
展开英文摘要原文
Transcription factors play a crucial role in the differentiation of tumor-specific CD8 + T cells and significantly influence their capacity to repress tumors.
Here, we present a protocol for creating a transcription regulator inhibitor of DNA 3 (ID3) conditional knockout mouse in tumor-specific CD8 + T cells, induced by tamoxifen.
We describe steps for examining the impact of ID3 deficiency on the differentiation of tumor-specific memory CD8 + T cells (Ttsm) and progenitors of exhausted CD8 + T cells (Tpex) in tumor-draining lymph nodes through a co-adoptive transfer assay. For complete details on the use and execution of this protocol, please refer to Ran et al. 1 .
论文信息
- 作者
- Xu L、Wang Y、Ran M、Liu W、Ye L、Huang Q
- 第一作者单位
- Provincial Key Laboratory of Immune Regulation and Immunotherapy, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, China.China
- 通讯作者单位
- Institute of Immunological Innovation and Translation, Chongqing Medical University, Chongqing, China. Electronic address: huangqizhaotcell@cqmu.edu.cn.China
- 期刊
- STAR protocols2025 Jun 20
- 原文标识
- PubMed 40138318 · DOI 10.1016/j.xpro.2025.103703
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