弥漫大 B 细胞淋巴瘤中临床预后因素、微血管密度及 TIL（肿瘤浸润淋巴细胞）与 CD47 和 SIRPα 表达的关系

CD47 interacts with signal regulatory protein alpha (SIRP ) on macrophages to deliver an anti-phagocytic signal, enabling tumor cells to evade immune destruction.

This study explores the relationship between CD47 and SIRP expression and key clinical prognostic factors, microvascular density (MVD), and tumor-infiltrating lymphocytes (TIL) in Diffuse Large B Cell Lymphoma (DLBCL) cases.

We analyzed tissue samples from 122 DLBCL cases using tissue microarray (TMA) blocks and immunohistochemical staining for CD47, SIRP , CD31, and CD3. CD47 expression was scored using the Allred scoring system, and SIRP expression was quantified based on the percentage of positive membranous and cytoplasmic expression.

Clinical data, including IPI scores, relapse rates, and gene expression profiles, were correlated with the immunohistochemical findings. CD47 expression score 6 was significantly associated with the DLBCL-ABC phenotype (p = 0. 029), higher IPI scores (p = 0. 020), and increased relapse rates (p = 0. 021). High SIRP expression ( 25 % staining) was also linked to the ABC phenotype (p = 0. 022) and frequent relapses (p = 0. 021).

Notably, cases with high microvascular density exhibited lower SIRP expression (p = 0. 013). There was no significant relationship between MVD and CD47 or other clinical prognostic factors.

Additionally, higher CD3-positive TIL percentages were inversely correlated with IPI scores (p = 0. 005), although no significant association was found between CD3 and CD47-SIRP . The study reveals that increased CD47-SIRP expression is partially linked to adverse prognostic indicators and reduced MVD in DLBCL cases.

These findings suggest that targeting the CD47-SIRP axis could offer a novel therapeutic approach in DLBCL, particularly for patients with poor prognostic features.