间皮素作为癌症免疫治疗的生物标志物和治疗靶点
Mesothelin as Biomarker and Therapeutic Target for Immunotherapy in Cancer.
癌症仍是一个关键的全球健康问题，原因在于发现晚、耐药和高死亡率。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
NK 和 NKT 样细胞上 Toll 样受体（TLR）的分析能否改善胃癌的诊断和治疗？
英文原题：Can the Analysis of Toll-like Receptors (TLR) on NK and NKT-like Cells Improve Gastric Cancer Diagnostics and Treatment?
查看英文原题
Can the Analysis of Toll-like Receptors (TLR) on NK and NKT-like Cells Improve Gastric Cancer Diagnostics and Treatment?
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中文摘要
方法
本研究纳入86例经组织学确诊的胃癌患者和30例健康志愿者。采集参与者外周血样本，通过流式细胞术和RT-qPCR评估NK和NKT样细胞上的TLR表达。使用来源于PBMC的NK和NKT样细胞的遗传物质评估TLR2、TLR3、TLR4和TLR9基因的表达。所得结果采用Mann-Whitney U检验和Kruskal-Wallis检验进行统计分析，并使用ROC曲线分析评估变量的预测能力。
结果
与健康志愿者相比，胃癌患者中TLR受体（TLR-2、TLR-3、TLR-4和TLR-9）的表达显著升高（p < 0.05）。TLR表达也因癌症亚型而异，在更晚期的GC亚型中观察到更高的表达。RT-qPCR分析显示，GC患者组中TLR基因表达显著增加。ROC曲线表明TLR具有较高的区分GC患者和健康个体的能力。
结论
胃癌患者NK和NKT样细胞上TLR的表达明显增加，尤其是在更晚期的肿瘤亚型中。结果提示，TLRs可能作为诊断和预后生物标志物，并代表GC免疫治疗的潜在靶点。然而，仍需进一步研究以确定TLRs在疾病进展中的功能作用及其用于个体化治疗的可能性。
展开英文摘要原文
Background/Objectives : The aim of this study was to determine the assessment of the percentage of NK and NKT-like cells expressing Toll-like receptors (TLR-2, TLR-3, TLR-4, and TLR-9) in patients with gastric cancer (GC) compared with healthy volunteers (HV) and to investigate differences according to cancer subtype.
We also assessed TLR gene expression by RT-qPCR to assess whether TLRs could be diagnostic and prognostic biomarkers. Methods : The study included 86 patients with histologically confirmed gastric cancer and 30 healthy volunteers. Peripheral blood samples were collected from the participants, and TLR expression on NK and NKT-like cells was assessed by flow cytometry and RT-qPCR. The expression of TLR2, TLR3, TLR4, and TLR9 genes was assessed using genetic material derived from NK and NKT-like cells sourced from PBMC. The obtained results were statistically analyzed using Mann-Whitney U and Kruskal-Wallis tests, and the predictive ability of variables was assessed using ROC curve analysis.
Results : A significantly higher expression of TLR receptors (TLR-2, TLR-3, TLR-4, and TLR-9) was found in patients with gastric cancer compared to healthy volunteers ( p < 0. 05). TLR expression also differed depending on the cancer subtype, and higher expression was observed in more advanced GC subtypes. RT-qPCR analysis showed significantly increased expression of TLR genes in the group of GC patients.
ROC curves indicate a high ability of TLRs to differentiate between GC patients and healthy individuals. Conclusions : The expression of TLRs on NK and NKT-like cells is clearly increased in patients with gastric cancer, especially in more advanced subtypes of the tumor. The results suggest that TLRs could potentially be used as diagnostic and prognostic biomarkers and represent potential targets for immune therapies in GC.
However, further studies are needed to determine the functional role of TLRs in disease progression and the possibility of their use in personalized treatment.
论文信息
- 作者
- Kos M、Bojarski K、Mertowska P、Mertowski S、Tomaka P、Zaborek-Łyczba M、Łyczba J、Dziki Ł
- 第一作者单位
- Department of Public Health, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland.Poland
- 通讯作者单位
- Department of Experimental Immunology, Medical University of Lublin, 4a Chodźki Street, 20-093 Lublin, Poland.Poland
- 期刊
- Cancers2024 Nov 17
- 原文标识
- PubMed 39594809 · DOI 10.3390/cancers16223854
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