间皮素作为癌症免疫治疗的生物标志物和治疗靶点
Mesothelin as Biomarker and Therapeutic Target for Immunotherapy in Cancer.
癌症仍是一个关键的全球健康问题，原因在于发现晚、耐药和高死亡率。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
胃癌中 RNF43 下调 PD-L1 表达增强 T 细胞的抗肿瘤活性
英文原题：PD-L1 expression downregulation by RNF43 in gastric carcinoma enhances antitumour activity of T cells.
查看英文原题
PD-L1 expression downregulation by RNF43 in gastric carcinoma enhances antitumour activity of T cells.
分数与星级只用于站内排序 —— 不代表疗效、安全性或个人适用性。
中文摘要
环指蛋白43（RNF43）是一种跨膜E3泛素连接酶，已被认为可能是胃癌治疗的潜在生物标志物，因为该蛋白可增加肿瘤细胞凋亡并抑制细胞增殖。RNF43在细胞免疫治疗中的作用仍不清楚。在此，我们旨在探讨RNF43在胃癌细胞系中的表达水平及其在细胞免疫治疗中的作用。分析了胃癌细胞系中RNF43与PD-L1的表达水平及其相关性。在胃癌细胞系中，PD-L1的表达与RNF43的表达呈负相关。RNF43与PD-L1相互作用，在胃癌细胞系中增强PD-L1的K48连接和K63连接泛素化。此外，胃癌细胞系中RNF43的表达可增强T细胞的抗肿瘤活性。总之，本研究揭示RNF43可抑制PD-L1表达，从而增强细胞免疫治疗的抗肿瘤活性。
展开英文摘要原文
Ring finger protein 43 (RNF43), a transmembrane E3 ubiquitin ligase, has been indicated to be a potential biomarker for gastric cancer treatment, as this protein increases tumour cell apoptosis and suppresses cellular proliferation. The role of RNF43 in cellular immunotherapy remains unclear.
Herein, we aimed to explore the expression level of RNF43 in gastric cancer cell lines and its role in cellular immunotherapy. The expression level of RNF43 and PD-L1 and their correlation in gastric cancer cell lines were analysed. The expression of PD-L1 was negatively correlated with that of RNF43 in gastric cancer cell lines. RNF43 interacted with PD-L1 to augment both K48- and K63-linked ubiquitination of PD-L1 in gastric cancer cell lines.
In addition, RNF43 expression in gastric cancer cell lines could enhance the antitumour activity of T cells.
In conclusion, this study reveals that RNF43 can inhibit PD-L1 expression to enhance the antitumour activity of cellular immunotherapy.
论文信息
- 作者
- Yang J、Wei M、Liu X、Shao X、Yan J、Liu J、Wen J、Zhang X
- 单位
- Department of Gastroenterology, the First Medical Center of Chinese PLA General Hospital, Beijing, China.China
- 期刊
- Scandinavian journal of immunology2023 Jun
- 原文标识
- PubMed 39007965 · DOI 10.1111/sji.13268