不适合移植的大 B 细胞淋巴瘤二线使用 axicabtagene ciloleucel：ALYCANTE 最终分析
Second-line axicabtagene ciloleucel in large B-cell lymphoma ineligible for transplantation: ALYCANTE final analysis.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
慢性淋巴细胞白血病的 Eμ-TCL1 过继转移小鼠模型
英文原题：Eμ-TCL1 adoptive transfer mouse model of chronic lymphocytic leukemia.
查看英文原题
Eμ-TCL1 adoptive transfer mouse model of chronic lymphocytic leukemia.
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中文摘要
尽管慢性淋巴细胞白血病（CLL）是西方世界最常见的成人白血病，但它仍然是一种危及生命且无法治愈的疾病。开发新疗法的努力高度依赖于是否有合适的用于临床前测试的小鼠模型。Eμ-TCL1 小鼠模型是研究 CLL 病理生物学和治疗反应最成熟的临床前方法，众多研究支持其与该恶性肿瘤最具侵袭性形式的相似性。与转基因 Eμ-TCL1 模型相比，在免疫健全的 C57BL/6 小鼠中采用 Eμ-TCL1 衍生脾细胞的过继转移，可产生一个相对快速（例如，与转基因模型中的数月相比，白血病在数周内发展）且可靠的模拟 CLL 的模型。在本章中，我们希望为读者提供一个经过彻底优化、详细且全面的方案，以便在其研究中使用过继转移 Eμ-TCL1 模型。
展开英文摘要原文
Despite being the most common adult leukemia in the western world, Chronic Lymphocytic Leukemia (CLL) remains a life-threatening and incurable disease. Efforts to develop new treatments are highly dependent on the availability of appropriate mouse models for pre-clinical testing. The Eμ-TCL1 mouse model is the most established pre-clinical approach to study CLL pathobiology and response to treatment, backed by numerous studies highlighting its resemblance to the most aggressive form of this malignancy.
In contrast to the transgenic Eμ-TCL1 model, employing the adoptive transfer of Eμ-TCL1-derived splenocytes in immunocompetent C57BL/6 mice results in a comparably rapid (e. g. , leukemic development within weeks compared to months in the transgenic model) and reliable model mimicking CLL. In this chapter, we would like to provide readers with a thoroughly optimized, detailed, and comprehensive protocol to use the adoptive transfer Eμ-TCL1 model in their research.
论文信息
- 作者
- Fernandez Botana I、Gonder S、Klapp V、Moussay E、Paggetti J
- 第一作者单位
- Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.Italy
- 通讯作者单位
- Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg. Electronic address: jerome.paggetti@lih.lu.Italy
- 期刊
- Methods in cell biology2024
- 原文标识
- PubMed 38880520 · DOI 10.1016/bs.mcb.2024.03.012