CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
英文原题：Apolipoprotein E is a prognostic factor for pancreatic cancer and associates with immune infiltration.
Apolipoprotein E is a prognostic factor for pancreatic cancer and associates with immune infiltration.
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APOE 可能通过调控肿瘤微环境中免疫细胞的浸润来影响胰腺癌的发生与发展。
胰腺导管腺癌（PDAC）中载脂蛋白 E（APOE）的表达水平及其对患者预后的影响尚不明确，APOE 对 PDAC 患者免疫状态的作用也未得到阐明。
从 TCGA 和 GETx 数据库获取胰腺癌数据。纳入 2012 至 2021 年在嘉兴学院附属第二医院接受胰腺手术的 PDAC 患者，记录临床病理资料、测定血浆 APOE 水平并收集组织样本，制备组织微阵列。通过 APOE 和 CD4 染色计算免疫反应评分（IRS）。分析并比较胰腺癌患者血浆和肿瘤组织中 APOE 表达，并评估血浆 APOE、组织 APOE 与临床病理特征之间的相关性。采用 Kaplan-Meier 生存分析和 Cox 多变量回归分析生存预后；进一步分析 APOE 表达与免疫标志物和免疫细胞的相关性，并使用 TISCH 网站进行 APOE 在不同细胞中的单细胞分布分析。
胰腺癌患者肿瘤组织中 APOE 高表达，血浆 APOE 高水平与不良预后相关。女性、高级别疾病患者及胰头癌患者血浆 APOE 水平较高。肿瘤组织 APOE 高表达与良好预后相关。胰腺癌微环境中的 APOE 主要由单核巨噬细胞表达。肿瘤微环境 APOE 水平与 CD8A、PDCD1、GZMA、CXCL10 和 CXCL9 等免疫标志物呈正相关。APOE 可促进肿瘤微环境中 CD4+ T 细胞或树突状细胞浸润。
APOE 可能通过调节肿瘤微环境免疫细胞浸润，影响胰腺癌的发生和发展。
The expression level of apolipoprotein E (APOE) in pancreatic ductal adenocarcinoma (PDAC) and its effect on the prognosis of PDAC patients are not clear. The effect of APOE on the immune status of patients with PDAC has not been elucidated.
We obtained pancreatic cancer data from the TCGA and GETx databases. Patients with PDAC who underwent pancreatic surgery at the Second Affiliated Hospital of Jiaxing University between 2012 and 2021 were included. Clinical pathological data were recorded, plasma APOE levels were measured, and tissue samples were collected. A tissue microarray was generated using the collected tissue samples. APOE and CD4 staining was performed to determine immunoreactive scores (IRSs). The expression of APOE in the plasma and tumour tissues of pancreatic cancer patients was analysed and compared. The correlations between plasma APOE levels, tissue APOE levels and clinicopathological characteristics were analysed. Survival prognosis was analysed using Kaplan-Meier survival analysis and Cox multivariate regression analysis. The correlations between APOE expression levels and immune biomarkers and immune cells were further analysed. Single-cell analysis of APOE distribution in various cells was performed on the TISCH website.
APOE was highly expressed in the tumour tissue of pancreatic cancer patients, and high plasma APOE levels were associated with poor prognosis. Females, patients with high-grade disease and patients with pancreatic head carcinoma had high plasma APOE levels. High APOE expression in tumour tissues was associated with good prognosis. Mononuclear macrophages in the pancreatic cancer microenvironment primarily expressed APOE. APOE levels positively correlated with immune biomarkers, such as CD8A, PDCD1, GZMA, CXCL10, and CXCL9, in the tumour microenvironment. APOE promoted CD4 + T cell or dendritic cell infiltration in the tumour microenvironment.
APOE may affect the occurrence and development of pancreatic cancer by regulating the infiltration of immune cells in the tumour microenvironment.
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