人喉鳞状细胞癌中增多的肿瘤浸润浆细胞样树突状细胞高表达 PD-L2 并影响 CD8(+) T 淋巴细胞浸润

The infiltration and prognostic significance of tumor-infiltrating plasmacytoid dendritic cells (TI-pDC) have been elucidated in various human solid cancers.

However, the infiltrating patterns and functional importance of TI-pDC in laryngeal squamous cell carcinoma (LSCC) remain unknown. In this study, flow cytometric analyses were conducted to characterize the infiltration of dendritic cells and T lymphocytes, along with their respective subgroups in tumor tissues (TT), para-carcinoma tissues (PT), and peripheral blood (PB) from LSCC patients.

Immunohistochemical staining for CD4 and CD8, as well as immunofluorescence staining for CD123, were performed on serial tissue sections to investigate the co-localization of TI-pDC and tumor-infiltrating T lymphocytes (TIL) within the tumor microenvironment (TME).

Our results demonstrated significantly lower percentages of all three DC subsets in PB compared to TT and PT.

Notably, the pDC percentage was markedly higher in TT than in PT.

Moreover, TI-pDC percentage was significantly elevated in N + stage patients compared to those with N0 stage. The results of survival analysis consistently demonstrated that high levels of TI-pDC infiltration were indicative of a poor prognosis.

Further investigation revealed a significant negative correlation between TI-pDC and CD8 + TILs; notably, pDCs expressed an inhibitory surface molecule PD-L2 rather than PD-L1 within PT. Collectively, our findings suggest that increased TI-pDC is associated with adverse outcomes in LSCC patients while exhibiting an inhibitory phenotype that may play a crucial role in suppressing CD8 + TILs within LSCC tumors. These results highlight the potential therapeutic strategy targeting PD-L2 + pDCs for immunotherapies against LSCC.