CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
精氨酸连接的 HPV 相关 E7 展示细菌源性外膜囊泡作为一种强效的抗原特异性癌症疫苗
英文原题：Arginine-linked HPV-associated E7 displaying bacteria-derived outer membrane vesicles as a potent antigen-specific cancer vaccine.
查看英文原题
Arginine-linked HPV-associated E7 displaying bacteria-derived outer membrane vesicles as a potent antigen-specific cancer vaccine.
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研究概要
精氨酸包覆策略的简便性彰显了免疫刺激性 OMV 的多功能性，可广泛用于个性化细菌免疫治疗应用。
研究思路结论见上方概要
基于细菌的癌症治疗已展示出对抗肿瘤的创新策略。近期研究聚焦于革兰氏阴性菌外膜囊泡（OMVs）作为一种新型癌症免疫治疗策略，因其作为多功能载体的固有特性。
在此，我们开发了一种展示人乳头瘤病毒（HPV）相关E7抗原的沙门氏菌来源OMV疫苗，利用聚(L-精氨酸)细胞穿透肽（CPP）增强HPV16 E7（aa49-67）H-2 Db与OMV的亲和力，命名为SOMV-9RE7。
由于OMV固有的免疫原性特性，SOMV-9RE7通过抗原呈递细胞摄取和抗原交叉呈递有效激活适应性免疫。接种工程化OMV显示出即时肿瘤抑制和肿瘤反应性浸润免疫细胞的募集。
展开英文摘要原文
Bacteria-based cancer therapy have demonstrated innovative strategies to combat tumors. Recent studies have focused on gram-negative bacterial outer membrane vesicles (OMVs) as a novel cancer immunotherapy strategy due to its intrinsic properties as a versatile carrier. METHOD: Here, we developed an Human Papillomavirus (HPV)-associated E7 antigen displaying Salmonella-derived OMV vaccine, utilizing a Poly(L-arginine) cell penetrating peptide (CPP) to enhance HPV16 E7 (aa49-67) H-2 Db and OMV affinity, termed SOMV-9RE7.
Due to OMV's intrinsic immunogenic properties, SOMV-9RE7 effectively activates adaptive immunity through antigen-presenting cell uptake and antigen cross-presentation. Vaccination of engineered OMVs shows immediate tumor suppression and recruitment of infiltrating tumor-reactive immune cells.
The simplicity of the arginine coating strategy boasts the versatility of immuno-stimulating OMVs that can be broadly implemented to personalized bacterial immunotherapeutic applications.
论文信息
- 作者
- Wang S、Chen CC、Hu MH、Cheng M、Tu HF、Tsai YC、Yang JM、Wu TC
- 第一作者单位
- Department of Pathology, Johns Hopkins University School of Medicine, 1550 Orleans Street, CRB II 307, Baltimore, MD, 21287, USA.United States
- 通讯作者单位
- Department of Pathology, Johns Hopkins University School of Medicine, 1550 Orleans Street, CRB II 307, Baltimore, MD, 21287, USA. chung2@jhmi.edu.United States
- 文献类型
- 非美国政府资助研究 · 美国 NIH 资助研究
- 期刊
- Journal of translational medicine2024 Apr 22
- 原文标识
- PubMed 38649894 · DOI 10.1186/s12967-024-05195-7
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