CXCR6 定义了用于儿童 B 细胞急性淋巴细胞白血病过继性细胞转移治疗的 CD4(+) 细胞毒性 T 细胞谱系的治疗亚型

The potential of cytotoxic CD4 + T cells and tissue resident memory T cells (Trm) in achieving adult leukemia remission have been highlighted [1,2].

We hypothesized that CXCR6 could serve as a marker for cytotoxic CD4 + Trm cells in the bone marrow (BM) of pediatric B-ALL patients. Flow cytometry (FCM) and published single cell RNA sequencing (scRNA-seq) datasets were employed to characterize CXCR6 + CD4 + T cells in the BM and peripheral blood (PB) of pediatric B-ALL patients and healthy donors. FCM, scRNA-seq and co-culture were utilized to explore the cytotoxicity of CXCR6 + CD4 + T cells in vitro based on in vitro induction of CXCR6 + CD4 + T cells using tumor antigens and peripheral blood mononuclear cells (PBMCs).

The ssGSEA based on the cell markers identified according to the in vivo scRNA-seq data, the TARGET-ALL-P2 datasets, and integrated machine learning algorithm were employed to figure out the key cells with prognostic values, followed by simulation of adoptive cell transfer therapy (ACT).

Integrated machine learning identified the high-risk cells for disease free survival, and overall survival, while simulation of ACT therapy using CXCR6 + CD4 + T cells indicated that CXCR6 + CD4 + T cells could remodel the bone marrow microenvironments towards anti-tumor. Based on the expression of genes involved in formation of resident memory T cells, CXCR6 is not a marker of resident memory CD4 + T cells but defines therapeutic subtypes of CD4 + cytotoxic T cell lineage for pediatric B-ALL.