CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
源自健康供者外周血单个核细胞的抗原特异性 T 细胞的分析与开发
英文原题：Analysis and Development of Antigen-specific T Cells Derived from Peripheral Blood Mononuclear Cells of Healthy Donors.
查看英文原题
Analysis and Development of Antigen-specific T Cells Derived from Peripheral Blood Mononuclear Cells of Healthy Donors.
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研究概要
利用 CFSE 染色鉴定抗原特异性 TCR 是开发有效免疫疗法的可靠方法。鉴定出的 CMV 或 EBV 特异性 TCR 可用于过继性细胞疗法以治疗癌症。
研究思路结论见上方概要
使用抗原特异性T细胞的过继性细胞疗法是一种有前景的癌症患者治疗方式。已知有多种方法可分离特异性T细胞并鉴定相应的T细胞受体（TCR）序列。本研究旨在从使用羧基荧光素琥珀酰亚胺酯（CFSE）标记增殖活化T细胞所分离的T细胞中鉴定抗原特异性TCR。
CFSE染色的健康供者外周血单个核细胞（PBMCs）用巨细胞病毒（CMV）或EB病毒（EBV）肽处理七天。随后，分离出CFSE染色减弱而增殖的T细胞，并对分离的T细胞进行单细胞VDJ测序，以鉴定抗原特异性TCR。
作为抗原特异性 TCR 候选，针对 CMV 抗原选择了十个 TCR 克隆，针对 EBV 抗原选择了五个 TCR 克隆。通过 NFAT-luciferase、IFN-ELISA 和细胞毒性试验，确认了十个 CMV TCR 转导的 T 细胞和一个 EBV TCR 转导的 T 细胞对负载 CMV 或 EBV 肽的 T2 细胞的反应性。
展开英文摘要原文
CFSE stained healthy donor peripheral blood mononuclear cells (PBMCs) were treated with cytomegalovirus (CMV) or Epstein-Barr virus (EBV) peptides for seven days. Then, proliferating T cells with decreased CFSE staining were isolated and single cell VDJ sequencing was performed on isolated T cells to identify antigen-specific TCRs.
As antigen-specific TCR candidates, ten TCR clones were selected for the CMV antigen and five for the EBV antigen. The reactivity of ten CMV TCR-transduced T cells and one EBV TCR-transduced T cell toward T2 cells pulsed with CMV or EBV peptide was confirmed via NFAT-luciferase, IFN- ELISA, and cytotoxicity assays.
Identification of antigen-specific TCRs with CFSE staining is a valid method for the development of effective immunotherapy. The identified CMV- or EBV-specific TCRs can be used for adoptive cell therapy to treat cancer.
论文信息
- 作者
- Kim J、Jung S、Jeong BK、Kim KA、Jisu J、Bang WS、Ham B、Kim JY
- 第一作者单位
- Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.South Korea
- 通讯作者单位
- Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; backlila@gmail.com.South Korea
- 期刊
- Anticancer research2024 Apr
- 原文标识
- PubMed 38537976 · DOI 10.21873/anticanres.16934