肿瘤细胞治疗研究
英文原题：PD-L1 Expression in Colorectal Carcinoma Correlates with the Immune Microenvironment.
PD-L1 Expression in Colorectal Carcinoma Correlates with the Immune Microenvironment.
本研究表明，在东印度队列的CRC中，PD-L1的频率相对较低。本研究中的免疫细胞浓度是使用基于图像分析的客观化方法计算的。肿瘤细胞中PD-L1表达与肿瘤浸润免疫细胞的显著相关性表明其在CRC病理生物学中的关键作用，特别是通过调节TIME。考虑到PD-L1在多种恶性肿瘤中的治疗意义，它可能是部分病例的关键治疗靶点之一。
这是一项基于医院的横断面观察性研究。PD-L1表达在蛋白质水平通过免疫组织化学评估，在mRNA水平通过qRT-PCR评估。免疫细胞标志物（CD4、CD8、CD20、FOXP3和CD163）使用ImageJ Fiji平台进行判读。
在104例病例中，21%为PD-L1阳性，且在右侧CRC中更为常见。PD-L1阳性病例显示所有T细胞亚群（CD4+、CD8+和FOXP3+）浓度显著更高，并观察到CD20+ B细胞和CD163+巨噬细胞。PD-L1表达与临床特征、病理亚型、分级或分期、错配修复状态（熟练 vs 缺陷）以及生存之间未见统计学显著性。
INTRODUCTION/BACKGROUND: Colorectal carcinoma (CRC) is a common malignancy, with its diverse clinical, pathological, and molecular features. The immune microenvironment of a tumor comprises of interplay between various cells and molecules, and has a significant role in deciding the tumor behavior and overall prognosis. PD-L1 (programmed cell death ligand-1) has been implicated in the regulation of the tumor immune microenvironment (TIME). There is limited data regarding the correlation of PD-L1 expression with immune cell profile in CRCs, especially in the Indian setting. The study aimed to assess the PD-L1 expression in CRC tumor cells and its association with TIME, mismatch repair (MMR), and various other clinicopathological parameters. METHODS: This is a hospital-based, cross-sectional observational study. PD-L1 expression was assessed at the protein level by immunohistochemistry and mRNA level by qRT-PCR. Immune cell markers (CD4, CD8, CD20, FOXP3, and CD163) were interpreted using the ImageJ Fiji platform. RESULTS: Of the 104 cases, 21% were PD-L1 positive and were more common in right-sided CRCs. PD-L1 positive cases showed significantly higher concentrations of all T-cell subsets (CD4+ , CD8+ , and FOXP3+), CD20+ B-cells, and CD163+ macrophages were noted. No statistical significance was seen between PD-L1 expression with clinical profile, pathological subtype, grade or stage, mismatch repair status (proficient vs deficient), and survival. CONCLUSIONS: The present study showed a relatively lower frequency of PD-L1 in CRC from the Eastern Indian cohort. The immune cell concentration in the present study was calculated using image analysis-based objectivised methods. Significant correlation of PD-L1 expression in tumor cells with the tumor-infiltrating immune cells indicated its crucial role in the pathobiology of CRC especially by regulating the TIME. Considering the therapeutic implication of PD-L1 in various malignancies, it may be one of the crucial therapeutic targets in a proportion of cases.
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