CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
逆转录病毒转导人原代 T 细胞及后续实时 T 细胞介导的癌细胞溶解分析
英文原题：Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis.
查看英文原题
Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis.
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中文摘要
逆转录病毒转导是对难以转染的人类原代细胞进行基因工程改造的高效工具。在此，我们用肿瘤特异性T细胞受体转导人类原代T细胞。这为分析T细胞与癌细胞的相互作用（如使用xCELLigence技术进行细胞裂解分析）创建了有用的工具。
展开英文摘要原文
Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.
论文信息
- 作者
- Rahbech A、Debets R、Thor Straten P、Peeters MJW
- 第一作者单位
- Department of Oncology, National Center for Cancer Immune Therapy, University Hospital Herlev, Copenhagen, Denmark.Denmark
- 通讯作者单位
- Department of Oncology, National Center for Cancer Immune Therapy, University Hospital Herlev, Copenhagen, Denmark. marlies.peeters@regionh.dk.Denmark
- 期刊
- Methods in molecular biology (Clifton, N.J.)2024
- 原文标识
- PubMed 38070105 · DOI 10.1007/978-1-0716-3593-3_3