Fe(3)O(4)-SWCNT 工程化 M1 样巨噬细胞的过继转移用于磁共振成像和增强癌症免疫治疗

Macrophage-based tumor immunotherapy can effectively kill tumor cells in a direct manner when tumor specific antigens are idle or unknown.

However, the presence of M2-like tumor associated macrophages (TAMs) would limit the treatment efficiency.

Therefore, reversing the M2-like TAMs phenotype to regulate the immunosuppressive tumor microenvironment (TME) is crucial.

Herein, we proposed nano-sized ferroferric oxide/single wall carbon nanotubes composites (Fe 3 O 4 -SWCNT) to engineer the macrophages species for powerful cancer therapy. The synthesized Fe 3 O 4 -SWCNT revealed good magnetic resonance imaging (MRI) performance, which enabled in vivo tracking of macrophage mediated immunotherapy.

In addition, Fe 3 O 4 -SWCNT engineered M1-like macrophages (Fe 3 O 4 -SWCNT@M1) could maintain M1 phenotype, migrate to tumor cells and release nitric oxide (NO), reactive oxygen species (ROS) and tumor necrosis factor-α (TNF-α). A series of experimental results showed that Fe 3 O 4 -SWCNT@M1 could effectively promote the polarization of endogenous M2-like macrophages to M1-like macrophages, activate tumor immune response and inhibit tumor progression. This work is expected to provide a new vision for macrophage-based tumor immunotherapy.