PIK3CA 突变亚型描绘了胃癌中不同的免疫特征

PIK3CA mutations in cancer regulate tumour immunogenicity. Given that PIK3CA mutation subtypes influence therapeutic responses to AKT inhibitor and that H1047R mutation confers selective growth advantages after immunotherapy, we hypothesised that immune phenotypes may depend on PIK3CA mutation subtypes.

We investigated 133 gastric cancers (GCs) harbouring PIK3CA mutation [21 E542K (15. 8%), 36 E545X (27. 1%), 26 H1047X (19. 5%), and 46 others (34. 6%)]. Four patients (3. 0%) had a combination of mutations (E542K + E545K in 3 patients and E545K + H1047R in 1 patient). Epstein-Barr virus (EBV) and microsatellite instability (MSI) status, PD-L1 (programmed death-ligand 1) combined positive score (CPS), and stromal tumour-infiltrating lymphocytes (TILs) were assessed.

Concurrent genomic alterations, GeoMx digital spatial profiling (DSP), and OPAL multiplex immunohistochemistry (mIHC) were analysed, and correlation between the two assays was investigated. Of the 133 PIK3CA-mutant (PIK3CA m ) GCs, MSI-high GC was significantly frequent in the H1047X mutation subtype (p = 0. 005), while EBV positivity did not affect the mutation subtypes. There was no significant survival difference between the E542K, E545X, and H1047X subgroups.

However, in the subgroup analysis for EBV-positive GC, H1047X m GC showed a trend towards shorter survival than E542K and E545X m GC (p = 0. 090 and 0. 062). With DSP analysis, H1047X m GC showed elevated VISTA (p = 0. 0003), granzyme B (p < 0. 0001), CD4 (p = 0. 0001), and CD45 (p < 0. 0001) expression compared with the E542K m or E545X m GC subgroups, and only VISTA expression remained significant (p < 0.

0001) using OPAL mIHC. DSP and OPAL analyses showed a moderate correlation of CD4 (ρ = 0. 42, p = 0. 004) and CD8 (ρ = 0. 62, p < 0. 001) expression levels in a comparison of six antibodies. Immune-related protein expression levels were evident when classified by the three PIK3CA hotspot mutations, and H1047X m GC showed the highest immune-related protein expression compared with E542K m or E545X m GC.

Our results demonstrated distinct immune profiles in GC with PIK3CA hotspot mutations using GeoMx DSP and OPAL mIHC, and there was a correlation between the two multiplex platforms. © 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.