CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
从人肿瘤中分离 CD8+ TIL（肿瘤浸润淋巴细胞）及其通过单细胞免疫图谱和 multiome 表征的实验方案
英文原题：Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome.
查看英文原题
Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome.
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中文摘要
理解TIL（肿瘤浸润淋巴细胞）群体的异质性以及人类癌症中的免疫生物学是建立有效免疫疗法的关键。在此，我们建立了一套方案，用于通过单细胞 RNA-seq 配对 VDJ 分析和染色质结构来表征肿瘤中的 CD8 + TIL，包括肿瘤活检组织的解离。我们还为后续的荧光激活细胞分选（FACS）、单细胞封装、生物信息学分析和故障排除提供了指导。关于本方案使用和执行的完整细节，请参阅 Anadon 等人（2022）。
展开英文摘要原文
Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies.
Here, we have established a protocol for the characterization of CD8 + TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies.
We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022).
论文信息
- 作者
- Anadon CM、Zhang C、Wang X、Cen L、Conejo-Garcia JR、Yu X
- 第一作者单位
- Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL 33614, USA.United States
- 通讯作者单位
- Department of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL 33614, USA. Electronic address: xiaoqing.yu@moffitt.org.United States
- 文献类型
- 美国 NIH 资助研究
- 期刊
- STAR protocols2022 Sep 16
- 原文标识
- PubMed 36065294 · DOI 10.1016/j.xpro.2022.101649
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