不适合移植的大 B 细胞淋巴瘤二线使用 axicabtagene ciloleucel：ALYCANTE 最终分析
Second-line axicabtagene ciloleucel in large B-cell lymphoma ineligible for transplantation: ALYCANTE final analysis.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
NKCD56bright 细胞在监测慢性淋巴细胞白血病治疗期间进展中的预测作用
英文原题：Predictive role of NKCD56bright cells in monitoring the progression of chronic lymphocytic leukemia during treatment.
查看英文原题
Predictive role of NKCD56bright cells in monitoring the progression of chronic lymphocytic leukemia during treatment.
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研究概要
在 CIT 过程中，CLL 细胞的减少伴随着免疫细胞群体的变化，包括 T 细胞、NK 细胞和 NKT 样细胞。监测外周血中这些细胞群体的变化可能作为重要的预测和预后指标。
研究思路结论见上方概要
背景
慢性淋巴细胞白血病（CLL）的标准治疗在过去几年中发生了巨大变化。直到最近，CLL仍采用抗CD20单克隆抗体的化学免疫治疗（CIT）。尽管BTKi（布鲁顿酪氨酸激酶抑制剂）和BCL2抑制剂等新型药物在大多数治疗场景中已成为标准治疗，但CIT在CLL治疗中仍占有一席之地。有趣的是，关于其对CLL患者免疫系统的影响知之甚少。与CIT给药期间CLL细胞数量减少相反，细胞微环境受到的关注很少，而在治疗期间对其进行评估可能提供有关疾病进程和预后的额外信息，因此被设定为本研究的目的。
方法
采用流式细胞术评估20例CLL患者外周血（PB）中不同淋巴细胞群体及亚群在CIT前、中、后的表型。
结果
在R-FC（利妥昔单抗、氟达拉滨和环磷酰胺）和R-B（利妥昔单抗、苯达莫司汀）方案的CIT期间，所评估的淋巴细胞群体和亚群的大小显著减少。第一个疗程治疗28天后，观察到CLL细胞呈指数下降，其数量已降至治疗前观察数量的中位水平10%。T细胞、NK细胞、NKCD56dim、NKT样细胞和NKT样CD56dim细胞也呈指数下降。第二个疗程治疗后，观察到T、NK、NKCD56dim、NKT样细胞和NKT样CD56dim细胞数量下降，并保持稳定直至第六个疗程。然而，NKT样CD56bright细胞的数量下降至第三个疗程，随后增加。外周血中CLL细胞的数量与NKCD56bright细胞的数量相关，影响治疗反应。
展开英文摘要原文
RESULTS
During the CIT with R-FC (Rituximab, Fludarabine, and Cyclophosphamide) and R-B (Rituximab, Bendamustine) regimens, the sizes of the assessed populations and subpopulations of lymphocytes were dramatically reduced. Twenty-eight days after the first course of treatment, the exponential decrease of CLL cells was observed, and their number had declined to the median level of 10% of the numbers observed before the treatment. T cells, NK cells, NKCD56dim, NKT-like, and NKT-like CD56dim also decreased exponentially. After the second treatment course, a decline in the numbers of T, NK, NKCD56dim, NKT-like, and NKT-like CD56dim cells was observed, which were stable until the sixth treatment course. However, the number of NKT-like CD56bright cells decreased to the third course of treatment and then increased. The number of CLL cells in peripheral blood correlated with the number of NKCD56bright cells, influencing the treatment response.
CONCLUSIONS
Upon CIT, the reduction of CLL cells is accompanied by shifts in immune cell populations, T, NK, and NKT-like cells. Monitoring changes of those cell populations in the peripheral blood may serve as an important predictive and prognostic indicator.
论文信息
- 作者
- Blachnio K、Grygalewicz B、Woroniecka R、Rygier J、Pienkowska-Grela B、Rymkiewicz G、Kawiak J
- 第一作者单位
- Flow Cytometry Laboratory, Pathology and Laboratory Diagnostics Department, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.Poland
- 通讯作者单位
- Polish Academy of Sciences, Department: Nałęcz Institute of Biocybernetics and Biomedical Engineering, Warsaw, Poland. jkawiak@ibib.waw.pl.Switzerland
- 期刊
- Folia histochemica et cytobiologica2022
- 原文标识
- PubMed 36045602 · DOI 10.5603/FHC.a2022.0023