CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
英文原题：Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC.
Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC.
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2型先天淋巴细胞（ILC2）在胰腺导管腺癌（PDAC）的肿瘤发生中发挥重要作用。ILC2介导的肿瘤发生的一个重要方面是驻留ILC2的扩增以及同时募集外周ILC2。在此，我们描述了一种用于ILC2分离、富集和DiD标记的方案，以便在小鼠体内追踪ILC2。此外，我们描述了将ILC2过继转移至PDAC受体小鼠模型的步骤。关于本方案使用和执行的完整细节，请参阅Alam等人（2022）。
Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2.
Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 for in vivo tracking of ILC2s in the mouse.
Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
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