CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
英文原题：CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models.
CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models.
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本方案详细介绍了CRISPR辅助表位插入（CRISPitope）的操作流程，这是一种灵活的、用于生成表达模型CD8+ T细胞表位的肿瘤细胞的方法，所述表位与内源性编码的选定基因产物融合。经CRISPitope工程化改造的肿瘤细胞可被免疫学研究中广泛使用的T细胞受体转基因（TCRtg）CD8+ T细胞识别。利用接种了CRISPitope工程化肿瘤细胞的小鼠，研究人员可以探究T细胞免疫治疗靶抗原的选择如何影响治疗效果及耐药机制。有关本方案使用和执行的完整细节，请参阅Effern等人（2020）。
This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach for generating tumor cells expressing model CD8 + T cell epitopes fused to endogenously encoded gene products of choice. CRISPitope-engineered tumor cells can be recognized by T cell receptor-transgenic (TCRtg) CD8 + T cells that are widely used in immunology research.
Using mice inoculated with CRISPitope-engineered tumor cells, researchers can investigate how the choice of the target antigen for T cell immunotherapies influences treatment efficacy and resistance mechanisms. For complete details on the use and execution of this protocol, please refer to Effern et al. (2020).
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