CELL INTELLIGENCE · 肿瘤细胞治疗研究

Gastric cancer (GC) remains a major cause of cancer‑related mortality worldwide, and only a subset of patients achieves durable benefit from immune checkpoint blockade (ICB). This suggests that non‑genomic barriers within the tumor microenvironment (TME) substantially limit antitumor immunity. Increasing evidence indicates that tumor‑intrinsic glycolytic reprogramming and lactate accumulation contribute to this immune resistance. Oncogenic signaling, hypoxia‑inducible factor‑1α (HIF‑1α), phosphoinositide 3‑kinase/protein kinase B/mechanistic target of rapamycin (mTOR) pathways and noncoding RNA networks promote the expression of glycolytic enzymes and lactate transporters, including hexokinase 2, 6‑phosphofructo‑2‑kinase/fructose‑2,6‑biphosphatase 3 (PFKFB3), pyruvate kinase M2, lactate dehydrogenase A (LDHA) and monocarboxylate transporters, thereby establishing a glycolysis‑high, lactate‑rich TME. Within this metabolic niche, lactate functions as a bioactive mediator that impairs dendritic cell differentiation and cross‑priming, weakens cytotoxic T‑cell and natural killer‑cell activity, and promotes M2‑like macrophages and myeloid‑derived suppressor cells through hydroxycarboxylic acid receptor 1/G protein‑coupled receptor 81‑dependent signaling and histone lactylation. Cancer‑associated fibroblasts and mesenchymal stem/stromal cells further reinforce this state through glycolysis, lactate shuttling, cytokine secretion, extracellular matrix remodeling and exosome‑mediated transfer of glycolysis‑promoting noncoding RNAs. These interactions generate spatially organized immunometabolic niches characterized by lactate accumulation, stromal remodeling, abnormal angiogenesis and poor CD8 + T‑cell infiltration. The present review summarizes the molecular drivers of glycolytic reprogramming in GC, the mechanisms by which lactate‑centered crosstalk reshapes stromal and immune compartments, and emerging therapeutic strategies targeting LDHA/monocarboxylate transporter 4, PFKFB3, HIF‑1α/mTOR, epigenetic regulators and repurposed metabolic drugs in combination with programmed death‑1/programmed death‑ligand 1 blockade. It is also discussed how fluorine‑18 fluorodeoxyglucose positron emission tomography/computed tomography, radiomics, glycolysis‑ and lactylation‑related gene signatures, exosomal biomarkers and dynamic metabolic monitoring may support patient stratification and response prediction. Viewing selected GC subtypes through a glycolysis‑centered immunometabolic framework may help guide the rational integration of metabolic and immune interventions to overcome metabolically protected, ICB‑refractory disease.