肿瘤细胞治疗研究
英文原题：Myeloid-derived suppressor cells in anaplastic thyroid carcinoma: insights from prospective immune profiling and implications for targeted immunotherapy.
ATC微环境中富含免疫抑制性MDSC。这些发现支持以MDSC为重点的免疫调节作为ATC中有前景的辅助治疗。
间变性甲状腺癌（ATC）对免疫治疗的反应有限。本研究旨在表征ATC与分化型甲状腺癌（DTC）及甲状腺滤泡结节性疾病（TFND）相比的免疫细胞组成，并评估IFN-γ和TGF-β mRNA表达。
细胞毒性T淋巴细胞（CTLs）、调节性T细胞（TREGs）、NK 细胞、B淋巴细胞和髓源性抑制细胞（MDSCs）通过多色流式细胞术在前瞻性收集的ATC、DTC和TFND组织中进行分析。IFN-γ和TGF-β mRNA表达使用RT-PCR进行定量。
ATC 显示出显著高于 DTC 或 TFND 的 MDSC 浸润。与 DTC 相比，ATC 中 CTL 升高，而 TREG、NK 和 B 细胞较低。IFN-γ 和 TGF-β 表达无显著差异，尽管 DTC 和 ATC 中 IFN-γ 表达高于 TFND。
PURPOSE: Anaplastic thyroid carcinoma (ATC) exhibits limited responsiveness to immunotherapy. The current study characterises the immune cell repertoire in ATC compared with differentiated thyroid carcinoma (DTC) and thyroid follicular nodular disease (TFND) and evaluates IFN-γ and TGF-β mRNA expression. METHODS: Cytotoxic T lymphocytes (CTLs), regulatory T cells (TREGs), natural killer cells (NK), B lymphocytes and myeloid-derived suppressor cells (MDSCs) were analysed by multicolour flow cytometry in prospectively collected ATC, DTC and TFND tissues. IFN-γ and TGF-β mRNA expression was quantified using RT-PCR. RESULTS: ATC demonstrated significantly higher MDSC infiltration than DTC or TFND. CTLs were elevated in ATC relative to DTC, while TREGs, NK and B-cells were lower. IFN-γ and TGF-β expression did not differ significantly although IFN-γ expression was higher in DTC and ATC than in TFND. CONCLUSION: ATC microenvironment is rich in immunosuppressive MDSCs. These findings support MDSC-focused immunomodulation as a promising adjunct therapy in ATC.
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