肿瘤细胞治疗研究
英文原题：Immunohistochemical Evaluation of Basal and Luminal Markers in Bladder Cancer: A Study from a Single Institution.
我们的研究阐明了 BC 的分子特征，强调了 CD44 表达的预后重要性以及 TILs 在影响亚型特异性结局中的作用。
背景：膀胱癌（BC）具有显著分子异质性，影响预后和治疗效果。免疫组织化学（IHC）有助于识别分子亚型及其与临床病理特征的关系。 方法：研究对107名BC患者组织样本进行IHC分析，评估GATA3、CD44、CK5/6和CK20标志物表达。采用两种方法将肿瘤样本分为基底型和腔面型，并探讨标志物表达、分子亚型、临床病理特征及TIL之间的关系。 结果：多数样本表达GATA3和CD44；CD44与CK5/6、GATA3与CK20之间存在显著相关性。CD44和CD20表达与较差预后相关。此外，腔面型和基底型具有不同的TIL分布模式，并影响总生存期。基底型且TIL浸润低，以及腔面型且TIL浸润高，均与不良预后相关。 结论：本研究阐明了BC的分子特征，强调CD44表达的预后重要性及TIL对亚型特异性结局的影响。IHC有助于识别亚型并支持个体化治疗策略。
BACKGROUND: Bladder cancer (BC) presents significant molecular diversity, which affects both prognosis and treatment results. Immunohistochemistry (IHC) facilitates the identification of molecular subtypes and their relationships with clinicopathological features. METHODS: We performed an IHC analysis on tissue samples from 107 BC patients, evaluating the expression of markers GATA3, CD44, CK5/6, and CK20. We applied two methods to classify the tumor samples into basal and luminal subtypes. The relationships between these marker expressions, molecular subtypes, clinicopathological characteristics, and TILs were explored. RESULTS: Most samples showed the expression of GATA3 and CD44, with notable correlations found between CD44 and CK5/6 as well as GATA3 and CK20. CD44 and CD20 expression were linked to a poorer prognosis. Additionally, the luminal and basal subtypes had distinct TIL patterns, which influenced overall survival. A poor prognosis was associated with the basal subtype with low TIL infiltration and the luminal subtype with high TIL infiltration. CONCLUSIONS: Our study clarifies the molecular characteristics of BC, underlining the prognostic importance of CD44 expression and the role of TILs in influencing subtype-specific outcomes. IHC proves valuable in subtype identification and supports personalized treatment strategies.
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