CELL INTELLIGENCE · 肿瘤细胞治疗研究

The impact of the immune response on the therapeutical efficacy of neoadjuvant chemotherapy for breast cancer remains largely unknown. To characterize the role of regulatory T cells (CD4 + CD25 + CD127 low Treg), T lymphocyte subsets (CD3 + , CD4 + , CD4 + /CD8 + ) and NK cells in neoadjuvant chemotherapy, we investigated the correlation patterns of these immune cell subsets with the progression of breast cancer. A total of 120 breast cancer patients receiving neoadjuvant chemotherapy in Nanjing Maternal and Child Health Hospital from May 2019 to November 2021 were retrospectively collected as the breast cancer group, and 46 healthy women were selected as the control group. The number of regulatory T cells, T lymphocyte subsets and NK cells in the peripheral blood were analyzed by flow cytometry. Compared with the control group, CD3 + , CD4 + , CD4 + /CD8 + ratio and NK cells were significantly decreased in patients with breast cancer ( P < 0.05), while the levels of Treg and CD8 + cells were significantly increased ( P < 0.05). In addition, the status of the immune response among breast cancer patients at different clinical stages was obviously different. In higher tumor stages, the level of CD3 + , CD4 + , CD4 + /CD8 + ratio and NK cell were reduced, while the level of Treg and CD8 + T cells gradually increased. Furthermore, we found a lower percentage of CD3 + , CD4 + , CD4 + /CD8 + and NK cells in association with lymph node metastasis, accompanied by a higher number of CD8 + T cells. Interestingly, after treatment with neoadjuvant chemotherapy, the levels of Tregs, CD3 + , CD4 + and CD4 + /CD8 + ratio of patients were all upregulated compared with the levels before treatment, indicating the recovery of cytotoxic lymphocytes and a consolidation of the immunosuppressive microenvironment at the same time ( P < 0.05 ). Immune dysfunction is commonly observed in breast cancer patients, which is closely associated with tumor progression and lymph node metastasis. Neoadjuvant chemotherapy was found to highly influence the number of T lymphocytes and improve the immune function of T lymphocyte subsets in breast cancer patients. At the same time, as immunosuppressive cells, the proportion of Tregs (CD4 + CD25 + CD127 low Treg) also increased after treatment with neoadjuvant chemotherapy. Our results provide guidance for the development of new combination strategies during neoadjuvant chemotherapy to reverse the immunosuppressive microenvironment and achieve better clinical outcomes.