肿瘤细胞治疗研究
英文原题：Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip.
在此，我们提出了一种基于多重荧光筛选的方法，适用于在新型多腔微孔芯片中对二维或三维细胞培养物进行检测，能够直接评估药物或免疫细胞的细胞毒性效力。
在此，我们提出了一种基于多重荧光筛选的方法，用于在新设计的多腔微孔芯片中对二维或三维细胞培养物进行筛选，从而能够直接评估药物或免疫细胞的细胞毒性效力。我们建立了一个框架，涵盖细胞培养、肿瘤球体形成、荧光标记以及在芯片中直接以不同放大倍数对固定或活细胞进行成像，并结合数据分析与解读。该方法通过使用卵巢癌和非小细胞肺癌细胞进行药物细胞毒性筛选，以及使用来自健康供体的NK 细胞针对肾癌和卵巢癌肿瘤球体进行细胞毒性筛选得到了验证。这种微型化形式允许长期细胞培养、高效筛选以及小样本体积的高质量成像，使该方法在精准医学的个体化细胞毒性测试中具有广阔前景。
Here, we present a methodology based on multiplexed fluorescence screening of two- or three-dimensional cell cultures in a newly designed multichambered microwell chip, allowing direct assessment of drug or immune cell cytotoxic efficacy. We establish a framework for cell culture, formation of tumor spheroids, fluorescence labeling, and imaging of fixed or live cells at various magnifications directly in the chip together with data analysis and interpretation. The methodology is demonstrated by drug cytotoxicity screening using ovarian and non-small cell lung cancer cells and by cellular cytotoxicity screening targeting tumor spheroids of renal carcinoma and ovarian carcinoma with natural killer cells from healthy donors. The miniaturized format allowing long-term cell culture, efficient screening, and high-quality imaging of small sample volumes makes this methodology promising for individualized cytotoxicity tests for precision medicine.
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