肿瘤细胞治疗研究
英文原题：Mesenchymal stem cell‑derived exosomes loaded with 5‑Fu against cholangiocarcinoma in vitro.
与游离5 Fu组相比，5 Fu Exos组显著抑制了CCA细胞的活力（P<0.01），表明5 Fu Exos可作为CCA治疗的有效化疗药物。
胆管癌（CCA）是一种难治性恶性肿瘤，恶性程度高，早期无症状。近年来大量研究表明，外泌体是化疗药物的有效递送载体，可在体内外抑制肿瘤增殖和生长。为探讨载5-氟尿嘧啶（5-Fu）外泌体对CCA生长的抑制作用，本研究使用人骨髓间充质干细胞来源的外泌体，以及孵育法和超声法将5-Fu载入外泌体，在体外治疗CCA。结果表明，从间充质干细胞中分离的外泌体具有典型的外泌体特征。孵育法和超声法均成功将5-Fu载入外泌体（5-Fu-Exos），其中超声法的载药效率高于孵育法。与游离5-Fu组相比，5-Fu-Exos组显著抑制CCA细胞活力（P<0.01），表明5-Fu-Exos可作为治疗CCA的有效化疗药物。
Cholangiocarcinoma (CCA) is an intractable malignant tumour with a high degree of malignancy that is asymptomatic in the early stages. Exosomes have been shown in numerous studies in recent years to be effective delivery vehicles for chemotherapy drugs to suppress tumour proliferation and growth in vivo and in vitro . In order to explore the inhibition of 5 fluorouracil (5 Fu) loaded exosomes on CCA growth, the present study used human bone marrow mesenchymal stem cell derived exosomes, as well as incubation and sonication methods for 5 Fu loading into exosomes, to treat CCA in vitro . The findings demonstrated that exosomes isolated from mesenchymal stem cells have typical exosome characteristics. Both the incubation and sonication methods successfully loaded 5 Fu into the exosomes (5 Fu Exos), with the sonication method having a higher loading efficiency than the incubation method. When compared to the free 5 Fu group, the 5 Fu Exos group significantly inhibited the viability of CCA cells (P<0.01), indicating that 5 Fu Exos can be an effective chemotherapy drug for CCA treatment.
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