不适合移植的大 B 细胞淋巴瘤二线使用 axicabtagene ciloleucel：ALYCANTE 最终分析
Second-line axicabtagene ciloleucel in large B-cell lymphoma ineligible for transplantation: ALYCANTE final analysis.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
用唑来膦酸扩增的人外周血γδ T 细胞的特征
英文原题：Characteristics of Human Peripheral Blood γδ T Cells Expanded With Zoledronate.
查看英文原题
Characteristics of Human Peripheral Blood γδ T Cells Expanded With Zoledronate.
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研究概要
γδ T 细胞可能是一种有前景的癌症免疫治疗手段。
研究思路结论见上方概要
背景
本研究旨在探讨在唑来膦酸（ZOL）存在下体外扩增的人外周血γδ T细胞的特性。
方法
人外周血细胞在添加或不添加ZOL（作为磷酸抗原）的条件下，与IL-2和IL-15共培养，并通过流式细胞术评估其表型。扩增后的γδ T细胞转导CD19 CAR载体，并通过流式细胞术在体外和体内评估其细胞毒性。
结果
离体扩增并未妨碍活化受体的表达。有趣的是，ZOL促进了γδ T细胞Vδ1亚群中CD226（DNAM-1）、TRAIL和FAS-L的表达。含有CD19 CAR + γδ T细胞的扩增γδ T细胞在体内有效清除了B细胞淋巴瘤细胞。
展开英文摘要原文
MATERIALS AND METHODS
Human peripheral blood cells were cultured with IL-2 and IL-15 in the presence or absence of ZOL, which was added as a phospho-antigen, and their phenotypes were assessed by flow cytometry. Expanded γδ T cells were transduced with CD19 CAR vector, and the cytotoxicity was evaluated in vitro and in vivo by flow cytometry.
RESULTS
Ex vivo expansion did not hamper the expression of activating receptors. Interestingly, ZOL promoted the expression of CD226 (DNAM-1), TRAIL, and FAS-L in the Vδ1 subset of γδ T cells. Expanded γδ T cells containing CD19 CAR + γδ T cells removed B cell lymphoma cells effectively in vivo.
CONCLUSION
γδ T cells could be a promising immunotherapeutic for cancer.
论文信息
- 作者
- Kim M、Kim H、Han M、Hwang HJ、Kim H、Im HJ、Kim N、Koh KN
- 第一作者单位
- Asan Institute for Life Sciences and Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.South Korea
- 通讯作者单位
- Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Republic of Korea pedkkn@amc.seoul.kr naykim@amc.seoul.kr.South Korea
- 期刊
- Anticancer research2021 Dec
- 原文标识
- PubMed 34848457 · DOI 10.21873/anticanres.15422