CELL INTELLIGENCE · 肿瘤细胞治疗研究

In myeloma, the bone marrow (BM) stroma mediates tumor growth directly and indirectly through the alteration of BM niches. The mesenchymal and endothelial cell subsets altered in the interstitial BM and focal lesions (FL) of patients newly diagnosed with myeloma, as well as in the myeloma-supportive human bone of the SCID-hu mouse model, were identified using single-cell atlases and gene expression profiling. The mesenchymal compartment showed enriched cells reflecting matrix cancer-associated fibroblasts (CAF) and vascular CAF/pericytes in FL compared to interstitial BM and in myeloma interstitial BM compared to healthy donors. Patients with myeloma possessed inflammatory mesenchymal stem cell (MSC) subsets that expressed genes resembling various CAF, including antigen-presenting CAF and genes composing the diagnostic three-gene MSC score for myeloma. The vascular compartment in FL showed reduced expression of genes representing specialized bone-remodeling endothelial cells and upregulation of genes reflecting angiogenic endothelial cells. We identified stroma factor-expressing CYR61/CCN1+ myeloid cells that were detected in myeloma but not in donors' bones. CYR61/CCN1+ myeloid cells co-expressed CD14, and their numbers were lower in the interstitial BM of patients with high-risk versus low-risk disease, and rare in FL. These cells were enriched in the BM aspirate lipid layer. The SCID-hu model showed changes in mesenchymal and endothelial cell subsets resembling clinical FL, except for inflammatory mesenchymal cells, which were present in the model but suppressed in FL. Overall, this study provides a comprehensive assessment of the altered stroma in myeloma and identifies previously unappreciated microenvironmental cell subsets. Specimens and data were obtained from patients enrolled in our TT2-TT5 Total Therapy clinical trials (clincaltrials gov. Identifier: NCT00083551, NCT00081939, NCT00572169, NCT00734877, NCT00869232) before initiation of treatment.