CELL INTELLIGENCE · 肿瘤细胞治疗研究

Recombinant interleukin-33 (IL-33) inhibits tumor growth, but the detailed immunological mechanism is still unknown. IL-33-mediated tumor suppression did not occur in Batf3 -/- mice, indicating that conventional type 1 dendritic cells (cDC1s) play a key role in IL-33-mediated antitumor immunity. A population of CD103 + cDC1s, which were barely detectable in the spleens of normal mice, increased significantly in the spleens of IL-33-treated mice. The newly emerged splenic CD103 + cDC1s were distinct from conventional splenic cDC1s based on their spleen residency, robust effector T-cell priming ability, and surface expression of FCGR3. DCs and DC precursors did not express Suppressor of Tumorigenicity 2 (ST2). However, recombinant IL-33 induced spleen-resident FCGR3 + CD103 + cDC1s, which were found to be differentiated from DC precursors by bystander ST2 + immune cells. Through immune cell fractionation and depletion assays, we found that IL-33-primed ST2 + basophils play a crucial role in the development of FCGR3 + CD103 + cDC1s by secreting IL-33-driven extrinsic factors. Recombinant GM-CSF also induced the population of CD103 + cDC1s, but the population neither expressed FCGR3 nor induced any discernable antitumor immunity. The population of FCGR3 + CD103 + cDC1s was also generated in vitro culture of Flt3L-mediated bone marrow-derived DCs (FL-BMDCs) when IL-33 was added in a pre-DC stage of culture. FL-BMDCs generated in the presence of IL-33 (FL-33-DCs) offered more potent tumor immunotherapy than control Flt3L-BMDCs (FL-DCs). Human monocyte-derived DCs were also more immunogenic when exposed to IL-33-induced factors. Our findings suggest that recombinant IL-33 or an IL-33-mediated DC vaccine could be an attractive protocol for better tumor immunotherapy.