CELL INTELLIGENCE · 肿瘤细胞治疗研究

Inclusion Criteria:

* Age ≥18 and \<70 years, regardless of gender;
* T-ALL/LBL was diagnosed according to the criteria of NCCN Clinical Practice Guidelines for Acute Lymphocytic Leukemia (2020.v1) and T-cell Lymphoma Clinical Practice Guidelines (2020.v1);
* Patients diagnosed with AML with reference to the Guidelines for Diagnosis and Treatment of Adult Acute Myeloid Leukemia (2018 Edition) issued by the Health Commission;
* Cytology confirmed that the tumor cells were CD7 positive.
* Number of blasts in bone marrow ≥5% at screening (bone marrow morphology);
* Complies with the diagnosis of relapsed/refractory AML, including any of the following conditions according to China Guidelines for Diagnosis and Treatment of Relapsed/Refractory Acute Myeloid Leukemia (2021 Edition): a. Primary refractory patients who did not achieve CR after two cycles of standard induction chemotherapy; b. CR after consolidation chemotherapy, relapse within 12 months; c. Relapse 12 months after remission but ineffective after conventional chemotherapy; d. 2 or more relapses; e. Relapse after hematopoietic stem cell transplantation.
* Meet the diagnosis of relapsed/refractory T-ALL/LBL, including any of the following: a. Primary refractory patients who have not achieved complete response after two cycles of standard chemotherapy, or patients who have not achieved complete response after multi-line rescue chemotherapy; b. Relapse within \<12 months after complete remission or ≥12 months after complete remission and fail to achieve complete remission induced by 1 or more cycles of standard treatment; c. Relapse after hematopoietic stem cell transplantation or relapse after CAR-T therapy at the same target;
* Complies with diagnosis of other relapsed/refractory CD7 positive hematologic malignancies
* Creatine clearance \>60ml/min (Cockcroft and Gault formula); serum total bilirubin ≤3 times the upper limit of normal, serum ALT and AST ≤5 times the upper limit of normal range for patients without liver invasion;
* showing left ventricular ejection fraction (LVEF) ≥50%;
* Pulse oxygen saturation ≥92%;
* The estimated survival time is more than 3 months;
* score 0-2;
* Subjects or their legal guardians voluntarily participate in this trial and sign the informed consent form.

Exclusion Criteria:

Subjects who met any of the following criteria were excluded from the study：

* acute promyelocytic leukemia (APL);
* Presence of a genetic syndrome such as Fanconi's anemia, Kostmann's syndrome, Shwachman syndrome or any other known syndrome of bone marrow failure;
* Patients with uncontrolled active central nervous system leukemia (CNSL), i.e. cerebrospinal fluid grades CNS 2 and CNS 3;
* Patients who have received anti-tumor therapy before infusion should be excluded if any of the following conditions are met: a. Systemic chemotherapy (except for pretreatment) within 1 week; b. For those who have received monoclonal antibody therapy, the last time of monoclonal antibody infusion is less than 5 half-lives or 4 weeks (whichever is shorter) at screening; c. Received donor lymphocyte infusion (DLI) within 6 weeks;
* Presence of uncontrolled, serious, active infection at screening;
* Patients with a history of serious heart disease, including: severe cardiac insufficiency (subjects with cardiac insufficiency of Class III or IV according to the New York Heart Association (NYHA) cardiac function classification standard), myocardial infarction within 12 months or cardiac angioplasty or stenting, unstable angina pectoris, ECG indicating significant QT interval prolongation (\>480ms) or serious arrhythmia judged by the investigator;
* Previous craniocerebral trauma, disturbance of consciousness, epilepsy, cerebral ischemia, cerebral vascular hemorrhagic disease and other medical history, and within six months of the need for drug treatment;
* Patients with hepatitis B surface antigen (HBsAg) greater than 10E6 IU/mL, hepatitis C virus (HCV) antibody positive, human immunodeficiency virus (HIV) antibody positive, syphilis antibody test positive, EBER positive or EBV copy number greater than the upper limit of normal at screening;
* Patients who must use steroid hormones during CAR-T infusion (except for local or inhaled steroid hormones); subjects who are receiving systemic steroid therapy before screening and need long-term systemic steroid therapy during treatment according to the investigator's judgment (except for inhaled or local use);
* Subjects with autoimmune diseases requiring treatment, immunodeficient subjects, or subjects requiring immunosuppressive treatment;
* Patients with acute graft-versus-host disease (GvHD) or moderate-to-severe chronic GvHD within 4 weeks prior to screening;
* Patients with a history of allergy to any component of cell products;
* Pregnant, lactating females, and subjects (male or female) of childbearing potential who are unable to use effective contraception within 1 year after cell infusion; male subjects who plan to become pregnant within 1 year after cell infusion; female subjects or partners who plan to become pregnant within 1 year after cell infusion;
* Any condition that, in the opinion of the investigator, may increase the risk to the subject or interfere with the results of the trial.

主要终点：Overall Response Rate (ORR) · 1. The proportion of patients achieving CR (complete remission) or CRi (complete remission with incomplete hematologic recovery) for T-ALL/LBL. 2. The proportion of patients achieving CR, CRi, or PR (partial remission) for T-ALL/LBL. 3. The proportion of patients achieving CRc \[composite complete remission, including CR/CRh (complete remission with partial hematologic recovery)\] or CRi for AML. · Efficacy will be evaluated at Weeks 4, 8, and 12 following supCD7 CART cell infusion.
次要终点：Total MRD-negative response rate (MRD-ORR) in bone marrow after supCD7 CART cell infusion；Duration of remission (DOR)；Event-free survival (EFS)；Leukemia-free survival (LFS)；Proportion of patients undergoing hematopoietic stem cell transplantation (HSCT) in remission

• The efficacy of supCD7 CART cell therapy · EXPERIMENTAL · This study will employ supCD7 CART cells to treat CD7-positive relapsed or refractory hematologic malignancies, particularly in patients with AML and T-ALL/LBL, with the aim of improving the re-remission rate in AML and T-ALL/LBL and providing a new therapeutic option to enhance their long-term survival.

The aim of this study was to evaluate the safety and efficacy of supCD7 CART cells in the treatment of patients with relapsed/refractory CD7-positive hematologic malignancies. In this single-arm, open-label, single-center, Phase Ⅰ+Ⅱ clinical trial, two cohorts were set up: (1) relapsed and refractory AML cohort; and (2) relapsed and refractory T-ALL/LBL cohort. Each cohort was planned to enroll 4-12 patients. SupCD7 CART cells will be administered intravenously to explore the MTD of each cohort using a 3+3 dose escalation and rapid titration design.